2.3. Natural fermentation for isolation and screening of
autochthonous LAB from CW
An aliquot of the extracted CW was used to isolate autochthonous
LAB microflora from the coconut. A 200 ml aliquot of
CW was incubated at 37 °C for 48 h in aerobic conditions. In
order to isolate colony-forming units (CFU) of cultivable populations,
1 ml of naturally fermented CW was serially diluted
tenfold in 0.05 mole/l sodium citrate (Sigma) buffer, pH 7.5, and
100 l of each dilution were spread on plates of MRS (de Man
Rogosa Sharpe) agar (Acumedia, Baltimore, MD, USA) pH 5.4 in
triplicate and incubated at 37 °C for 48 h as described by De
Dea Lindner et al. (2008). More than 100 CFUs were isolated and