Freeze-dried Bacterial cellulose samples from the Hot water extract and Hestrin–Schramm culture medium were pressed manually using a Dake Press in to cylindrical wafers 2 cm in diameter and 0.05–0.1 cm thick. A silicon zero background plate was used to make sure that there were not any peaks resulting from the sample holder.The same sample holder position and sample holder (holder and silicon zero background plate) were used for both samples. Crystallinity index (C.I.) and percentage crystallinity (% Crystalline) of Bacterial cellulose samples were calculated as follows (Rosli, Ahmad, & Abdullah,2013):