Glyphosate is a herbicides classified in the group of phosphonase and has chemical name as N- (Phosphonomethyl) Glyphosate is widely use presently. However, huge amounts of glyphosate became crucial cause of chemical residues and also extend its half-life in soil. This research is aimed at separating bacteria that are effective in the glyphosate degradation used in the rehabilitation of agricultural area.
The 80 samples of soil and water from agriculture area were isolated by enrichment technical with increasing concentrate of glyphosate as 1, 3, 6 and 12 g / L and 19 isolates were able to grow. When increase the concentration of glyphosate as 15,18 and 20 g / L and measuring the rate of growth by turbidity. It was found that 10 isolates were selected. Consequently, the degradation of glyphosate was determined by quantified the amount by HPLC. The growth rate was measurement by the turbidity of culture. Five isolates were selected as the best glyphosate degrader in which WPTC28.41.9, WPTC28.41.7 and WPTC28.35.1 were the first 3 isolates which can degrade glyphosate as 52.17%, 49.3% and 47.5% respectively.
Further, the optimal of temperature, pH, and salinity of the isolate has also been investigated and revealed that an optimum temperature at 37 – 45ºC and was able to growth at a wide range of NaCl and pH. These promising glyphosate degrading isolates were tentatively identified biochemical and MALDI-TOF MS characterization as Klebsiella pneumoniae. This study indicates that Klebsiella pneumonia which isolated in this study could be useful in some areas where this herbicide is extensively used.