IC50 of DPPH scavenging activity of GMR96% and 70 % ethanolic extracts were (7,48 ± 0,19)% and (6.563 ±
0.311)%. These IC50 is higher, that’s meant less DPPH scavenging activity, than reported by Palakawong et al.
7
which was 5.94 μg/mL although they used 50% ethanol for the extraction. The different region of G.mangostana L
collection and degree of the fruit ripeness might play a role to cause it.
Similar results were concluded about different antioxidant activity in using various extraction solution for sample
extraction by Zarena and Udaya Sankar4
. This difference could be caused by different extraction capacity of each
solution to extract GMR polar active compund4
. Phenolic compound content of GMR extract such as tannin, alpha
mangostin, epicatechin was different if solvent extraction was different. Therefore DPPH and hydroxyl radical
scavenging and also lipid peroxidation inhibition activity was different in several extraction solution.Water extract
had stronger capacity than methanol extract whilemethanol extract had stronger capacitythan hexane extract9
. Our
study had the similar results with that ones.
Total antioxidant status/ total antioxidant capacity (mM Trolox) of these GMR extracts, fractions, and alpha
mangostin is shown in Figure 2.