The cows received an intravaginal P4 device containing
1.0 g of P4 (Cronipress; Biogenesis Bago, Curitiba, PR, Brazil)
along with 2 mg of estradiol benzoate intramuscular (2-mL
Bioestrogen; Biogenesis Bago) on the first day of the synchronization
protocol (Day 10; Fig. 1)