Sephadex LH-20 is a liquid chromatography media designed for molecular sizing of natural products such as steroids, terpenoids, lipids and low molecular weight peptides (up to 35 amino acid residues).
Depending on the chosen solvents, this media can also separate sample components by partitionbetween the stationary and mobile phases.
Sephadex LH-20 is useful at both analytical and industrial scale for the preparation of closely related molecular species. Due to the unique physico-chemical properties of this media, it can be used either during initial purification prior to
polishing by high performance ion exchange or reversed phase chromatography or as the final polishing step e.g. during the preparation of diastereomers.