2.4. Isolation, purification and conservation of strains
Ten to thirty colonies growing on TCBS agar media (TCBSB) were isolated in each sample. A total of 617 strains were finally isolated. Each strain was purified by streaking them two times on ZoBell media. A strain was considerate purified after a visual observation of the colony and microscope observation of the cells. The purified strains were conserved on screw cap bottles contained 10 ml of T1N1 agar media, which is recommended for Vibrios. The strains were conserved at 20–25C and subculturing, one time after 3 months of their isolation, for analysis.