The developed method was then tested by evaluating the uptake of APAP in the aquatic fungus, M. hiemalis as a preliminary investigation. We show that APAP was taken up in M. hiemalis making it a suitable candidate for mycoremediation of the xenobiotic in question, however, further studies are needed to gain more insight into the tolerance threshold of M. hiemalis against pharmaceuticals and mixtures there of and the longevity of the fungus with exposure.