Broccoli ethanolic extracts were prepared as described in Section 2.7 and the supernatants were used to measure antioxi-
dant capacity with DPPH and ABTS+ radicals. The DPPH assay was done according to the method described by Brand-Williams et al. (1995) with minor modifications. Test tubes containing 0, 50, 75, 100 and 150 mL of sample and ethanol to a final volume of 150 mL were prepared. After that, 1000 mL of a 59.2 mg Ll solution of the radical DPPH dissolved in ethanol solution were added. Samples were vortexed and incubated at 20 C for 90 min. The absorbance was measured at 515 nm (UV-Mini 1240 model, Shimadzu Corporation, Japan) and the equivalent mass of florets tissue required to consume 50% of the initial DPPH was calculated (EC50). The antioxidant capacity was expressed as EC50 1 per kilogram. Four replicates were analyzed per type of inflorescence and storage time.
Broccoli ethanolic extracts were prepared as described in Section 2.7 and the supernatants were used to measure antioxi-dant capacity with DPPH and ABTS+ radicals. The DPPH assay was done according to the method described by Brand-Williams et al. (1995) with minor modifications. Test tubes containing 0, 50, 75, 100 and 150 mL of sample and ethanol to a final volume of 150 mL were prepared. After that, 1000 mL of a 59.2 mg Ll solution of the radical DPPH dissolved in ethanol solution were added. Samples were vortexed and incubated at 20 C for 90 min. The absorbance was measured at 515 nm (UV-Mini 1240 model, Shimadzu Corporation, Japan) and the equivalent mass of florets tissue required to consume 50% of the initial DPPH was calculated (EC50). The antioxidant capacity was expressed as EC50 1 per kilogram. Four replicates were analyzed per type of inflorescence and storage time.
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