automatic fraction collector and every 2 samples were combined
to obtain 17 fractions at the end of extraction. The extraction was
performed in 3 replicates. From each extraction 4 ml was sampled
for bioassay, other 12 ml for NMR. All were dried under N2 and put
overnight in freeze drier before analysis. Concentration of the
extracts for the bioassay and NMR were adjusted to 1.4 mg/ml
DMSO and 5–10 mg/ml MeOD, respectively