abstract
To investigate the effects of exogenous Spd on proteomic changes under normal condition and NaCl
stress of 3 days in cucumber seedling leaves, a 2-DE gel electrophoresis and MALDI-TOF/TOF MS was
performed. A total of 63 differentially expressed proteins responded to salt stress or exogenous Spd
treatments, and they were all successfully identified by MALDI-TOF/TOF MS. Many changes were
observed in the levels of proteins involved in energy and metabolic pathways, protein metabolic, stress
defense, and other functional proteins. Increased salt tolerance by exogenous Spd would contribute to
higher expressions of proteins involved in the SAMs metabolism, protein biosynthesis, and defense
mechanisms on antioxidant and detoxification. Meanwhile, the regulation of Calvin cycle, protein folding
assembly and the inhibition of protein proteolysis by Spd might play important roles in salt tolerance.
This study provides insight that may facilitate a better understanding of the salt resistance by Spd in
cucumber seedlings.
2013 Elsevi