For the second, the 3-D analysis with skin replica was used.5,6 Skin replica of wrinkles was constructed. The average roughness of the skin surface, “Ra” (ISO, DIN), was calculated using a PRIMOS-compact (GFMesstechnik, Teltow, Germany). PRIMOS com- pact is an optical 3-D in vivo skin measuring system based on the stripe projection technique. A parallel stripe pattern is projected onto the measuring area via projection optics and displayed on the chip of a charge-coupled device matrix camera via recording optics. Ra value is calculated from height profile that is measured from the reflection of the parallel stripes. Reduction of Ra means reduction total area of wrinkles