The antioxidant activity of the extracts was measured on the
basis of scavenging activity of the stable DPPH (1,1-diphenyl-2-
picryl-hydrazyl) stable free radical. One milliliter of different
concentrations of extract solution and standard ascorbic acid were
taken in different vials. To this 5 ml of methanolic solution of DPPH
was added, shaken well and mixture was incubated at 37 C for
30 min. Absorbance was measured against methanol as blank at
517 nm using a UV–visible spectrophotometer. Absorbance of DPPH
was taken as control. Percent antiradical activity can be calculated
by using following formula. The analysis was carried out in triplicate