A simple random technique was used to sample 100 keyboard and mice from offices and internet cafés by swabbing their surfaces. All samples were contaminated with bacteria showing greater than 105 (>105) CFU by aerobic counting technique. Internet Cafés had higher counts than offices which were significant (P≤0.05) (Table I). Eight (8) different bacteria were isolated from the samples of which seven (7) were pathogenic and one Coagulase Negative Staphylococcus was non-pathogenic. Bacillus cereus was the highest isolate in both samples and sample areas whilst more P. mirabilis was the highest isolate in Internet cafés keyboard and K. pneumoniae office mice. The highest (85.71%) and the least (75.00%) pathogenic isolates were found in Internet café keyboards and mice respectively (Table II). For Gram Positive bacterial, B. cereus and S. aureus showed the highest susceptibility (37.5%) to the antibiotics applied (Table III). All Gram positive bacterial isolates showed 100% resistant to Ampicillin (AMP) (10µg), Cloxacillin (CXC) (5µg) and Penicillin (PEN) (10µg) but 100% susceptible to Gentamicin (GEN) (10µg) and Cotrimoxazole (COT) (25µg) respectively (Table IV). K. Pneumonia was the most resistant Gram negative isolate (75.0%), followed by P. mirabilis (65.0%) with E. coli the least (50.0%) (Table V). Gram negative bacterial isolates were 100% resistant to Ampicillin (AMP) (10µg) and Cefuroxime (CRX) (30µg) but 100% susceptible to Amikacin (AMK) (30µg) and Cotrimoxazole (COT) (25µg) (Table VI).