The elution of the lipase was performed with the same buffer at a flow rate of 18 ml/h.The flow rate was adjusted to 18ml/h. The protein elution profile was recorded spectrophotometrically at 280 nm. Fractions with lipase activity eluted from the column were pooled and con- centrated using a Vivaspin centrifugal concentrator. The purified enzyme was used for further biochemical characterization.