Th e p l an t sa m pl e s were cl ea n e d and s ter i li z e d . The pl a n t
ti ss u e s were cu t into sl i ce s of 0. 5– 1 mm , the sl ic es were soaked in
2% gl u t a ral d e hyd e for 12 h. The sl i ce s were rins ed with PB S buff er
(p H 6. 8 ) fi ve times, eac h t i me , res pe ct ively for 5 min, 10 mi n ,
15 min, 20 mi n , 30 mi n ; th e n th e se slices were d ehyd rated with a
se r i e s of co nc e n t rati o n s of ac e tone (3 0% ! 50% ! 70% ! 80 %
! 90% ! 10 0% ) ste p by ste p , the time of eac h s tep was 20 mi n ,
th e sl i ce s were soaked in iso a myl a ce t ate for rep la ce m e n t
rea ct i on for 2 time s, each ti m e was 20 mi n . Th e se initial sa m pl e s
were dried, co ated , and o b se r ved under th e sc a n ni n g e le ct ron
mi cr o sc o p e
Th e p l an t sa m pl e s were cl ea n e d and s ter i li z e d . The pl a n tti ss u e s were cu t into sl i ce s of 0. 5– 1 mm , the sl ic es were soaked in2% gl u t a ral d e hyd e for 12 h. The sl i ce s were rins ed with PB S buff er(p H 6. 8 ) fi ve times, eac h t i me , res pe ct ively for 5 min, 10 mi n ,15 min, 20 mi n , 30 mi n ; th e n th e se slices were d ehyd rated with ase r i e s of co nc e n t rati o n s of ac e tone (3 0% ! 50% ! 70% ! 80 %! 90% ! 10 0% ) ste p by ste p , the time of eac h s tep was 20 mi n ,th e sl i ce s were soaked in iso a myl a ce t ate for rep la ce m e n trea ct i on for 2 time s, each ti m e was 20 mi n . Th e se initial sa m pl e swere dried, co ated , and o b se r ved under th e sc a n ni n g e le ct ronmi cr o sc o p e
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