Capillary electrophoretic (CE) and high performance liquid chromatographic (HPLC) methods were developed
and optimized for the determination of antazoline (ANT) and tetrahydrozoline (TET) in ophthalmic
formulations. Optimum electrophoretic conditions were achieved using a background electrolyte of
20 mM phosphate buffer at pH 7.0, a capillary temperature of 25 ◦C, a separation voltage of 22 kV
and a pressure injection of the sample at 50 mbar for 17 s