MS basal medium (Murashige and Skoog, 1962) was used for the present study. Agar-gelled (0.8% agar) and without agar (liquid) MS medium was prepared with help of macro and micro nutrients,
amino acids, vitamins and sucrose (3%) as sources of carbon. Cultures were maintained at 25 ± 2 ◦C and 40–50 mol m−2 s−1 Spectral Flux Photon (SFP) light intensity under 12:12 h light:dark
photo regimes.