Preparation of inoculum
Spores were obtained from mycelium grown on a Malt Extract Agar (MEA; Merck
Ltd, Thailand) medium at 30 °C for 14 days and were collected by flooding the surface of
the plates with ~ 5 ml sterile saline solution (NaCl, 8.5 g/l water) containing Tween 80
(0.1 % v/v). After counting the spores, the solution was standardized. Mould growth was
observed after inoculation of 107 spores at the center of the plates (10 μl of a standardized
suspension 107 spores/ml).