Rough rice (10 g) from each lot was dehulled using a manually-operated, portable
dehuller (Rice Husker TR120, Kett Electric Laboratory, Tokyo, Japan). From each
lot, two sets of 100 brown rice kernels were randomly selected for scanning. The 100
brown rice kernels were positioned on a tray (152 mm × 100 mm × 20 mm) made from
a 2 mm-thick clear acrylic sheet (Plexiglass) such that no kernel was touching another
kernel. The tray was then placed on the scanner for imaging. Percent chalkiness was
measured as the percent of total projected area of 100 kernels. The chalk level for each
lot was the average of two measurements of 100 kernels.