Agar spot test experiments were conducted by
spotting 10 mL of an overnight lactic acid bacterial
culture onto the surface of a MRS agar plate and
incubating at 30 °C until good growth was evident
(24 h). Th ese plates were then overlaid with 8 mL
of soft agar (0.75% agar) seeded with 8 mL of a test
bacteria culture (approximately 107
stationary-phase
cells). Aft er overnight incubation at 37 °C, the plates
were examined for zones of inhibition in the test
bacteria