10 ml solution from each of the initially prepared botanical extracts (1gml-1) was transferred into 500ml volumetric flask using a pipette and the final volume was made to 500ml to obtain a solution with a concentration 20 mg/ml (2% w/v). From these solutions different dilutions were obtained by adding certain volume of botanical solution, certain volume of distilled water and 1ml of 1% (w/v) soap solution.