The 102 RILs were analysed by HPLC-MS (see section below) to assess SL abundance and sugar assays to assess the concentrations of sucrose, fructose and glucose (see section below) in order to determine which lines would be most informative. Eight RILs were selected, based on whether that line had high or low concentrations of sugar and SLs. The sample size was kept small to avoid fatigue in the consumer panel.