Porphyra pseudolinearis, which does not produce asexual spores such as archeospore, is
a commercially valuable Porphyra seaweed. We obtained a large amount of regenerable free cells of P.
pseudolinearia by gentle homogenization in seawater after culturing the thalli in nutrient-rich medium with
10 mM of allantoin at 15 °C and 20 °C. The obtained free cells were divided into three forms: globular, elliptic
and 2-celled. These free cells formed cell walls slowly compared to conchospores, and they directly
developed to thalli or filamentous germlings like conchocelis or conchosporangia. In particular, the
occurrence of thalli was remarkable in free cells obtained from the thalli cultured with 10 mM allantoin at
20 °C. Once the free cells developed to the thalli, they showed the same rates of growth to the thalli
generated from conchospores, and they grew to be more than 50 cm in blade length. This technique to
produce regenerable free cells from the Porphyra species that do not produce archeospores is useful for
vegetative propagation and breeding.
Porphyra pseudolinearis, which does not produce asexual spores such as archeospore, isa commercially valuable Porphyra seaweed. We obtained a large amount of regenerable free cells of P.pseudolinearia by gentle homogenization in seawater after culturing the thalli in nutrient-rich medium with10 mM of allantoin at 15 °C and 20 °C. The obtained free cells were divided into three forms: globular, ellipticand 2-celled. These free cells formed cell walls slowly compared to conchospores, and they directlydeveloped to thalli or filamentous germlings like conchocelis or conchosporangia. In particular, theoccurrence of thalli was remarkable in free cells obtained from the thalli cultured with 10 mM allantoin at20 °C. Once the free cells developed to the thalli, they showed the same rates of growth to the thalligenerated from conchospores, and they grew to be more than 50 cm in blade length. This technique toproduce regenerable free cells from the Porphyra species that do not produce archeospores is useful forvegetative propagation and breeding.
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