DPPH radical scavenging capacity
DPPH was dissolved in methanol to a concentration of 10
−4
mol/l.
DPPH radical scavenging capacity was estimated according toBlois
(1958) and Shyu and Hwang (2002)with slight modification. Two
milliliters of diluted extract was mixed with 2 ml of DPPH solution,
the mixture was kept in the dark for 60 min and the absorbance
at 517 nm was measured. The scavenging capacity was calculated as
(1−Ab/A0)×100% (where Abis the absorbance at 517 nm; A0refers
to the absorbance of DPPH without sample added at 517 nm).