It is 20 years since the Evidence Based Medicine Working Group published the first paper on evidence-based practice (EBP) introducing new teaching aspects in medical education [1]. Today, EBP is emphasized to increase the quality of care and patient safety in healthcare, and health professionals are expected to implement evidence into their daily clinical practice [2, 3]. EBP is identified as being one of five core competencies in health professional education [3]. Nursing staff, in particular registered nurses (RNs), are the largest health professional group in all sectors of healthcare [4]. The majority of RNs work in direct care of patients; assessing patients’ needs and making decisions on nursing interventions. RNs’ practice of EBP can be assumed to have a major impact on patients’ outcomes and patient safety. Hence, there is a potential to improve quality of care and patient safety by enhancing RNs’ practice of EBP. Interventions aiming to enhance RNs’ practice of EBP need to target the factors that are important for EBP.