Acetobacter pasteurianus FS1, isolated from industrial vinegar bioreactors, was kept frozen at 70 C in cryovials (30% v/v glycerol) used throughout the article. Two different media were used in this article: YG1 medium (1 g of yeast extract, 1 g of glucose
per 100 ml of distilled water), YG2 medium (0.5 g of yeast extract, 0.5 g of glucose, 0.04 g of MgSO4 and 0.06 g of KH2PO4 per 100 ml of distilled water)