Measurement
There are several methods for the determination of
acid and D-glucono-d-lactone. Among them,
isotachophoretic method (17) and hydroxamate method
(18) are the most commonly used ones for the determination of gluconic acid. The concentration of gluconic
acid is also determined by gas chromatography of their
trimethylsilyl (TMS) derivatives prepared according to
Laker and Mount (19) with inositol as internal standard.