Sensitivity and specificity.The detection limit for nested
PCR and real-time PCR corresponded to 10
−4
and 10
−3
dilu-tion of DNA of one S. stercoralisfilariform larva, respec-tively (Figures 1 and 2). As, except for S. stercoralis,no
amplification was detected by nested PCR and real-time
PCR from DNAs extracted from all the above mentioned
microorganisms, we considered both methods highly specific
forS. stercoralis.