2.4. Experimental design
Single factor experimental design was used to optimise the
extraction conditions and study the effects of the extraction temperature,
time, solvent loading and agitation speed on the extract
yield and sludge dewatering ability of the bio-flocculants. Six sets of
experiments were carried out to establish the most significant
factors affecting the extraction of bio-flocculant. The parameters
studied are presented in Supplementary Table S2.