(a) TLC fingerprint profile of n-hexane fraction of Vitex negundo leaves. (1) Lupeol standard; (2) and (3) n-hexane fraction; (4) β-sitosterol standard. (b) TLC densitometric chromatogram of n-hexane fraction of Vitex negundo at 525 nm. a: Lupeol standard; b: n-hexane fraction; c: β-sitosterol. (c) Structure of lupeol and β-sitosterol.