Filtration and sample preparation method was designed
based on samples accepted per day. Of the advantages
of PCR system, sensitivity [detection of target bacteria in
minimum concentration, specificity of this method for target
microorganisms, rate of doing test from the time of sample
collection until analysis completion (less than 4 h)], and the
ability to simultaneously detect multiple bacterial (including
general specific species and a number of target specific
pathogens) can be mentioned. With regard to the emerging
problem of living bacteria but not being able to be cultured
(VBNC) and their role in public health, the results of this study
indicated that the provided molecular methods may also
be able to detect VBNC bacteria[10]. On the other hand, from
the perspective of economic justification, according to the
conducted studies, costs of culture methods are higher than the
costs of PCR method.