Soxhlet and modified soxhlet extractions
Exhaustive extraction with methanol (250 mL) was performed in a soxhlet apparatus, the thimble of which contained a weighed portion of plant material (40 g). Continuous extraction was performed for about 22 h. The obtained extract was concentrated on a water bath and the yield was calculated. A weighed sample (40 g) of the powdered materials in the thimble were introduced in to DBSA which was connected with two distillation flasks through inverted Y shaped joints, as shown in Fig. 3. DBSA extraction Inverted Y-shaped joint was performed with 500 mL of methanol during 12 h