by sequential immersion in 5% hypochlorite for 1-2
minutes followed by 75% ethanol for 30 seconds. Surface sterilised segments
(4 segments / plate) were selected, plated out on water agar amended with 0.5
gm streptomycin, 2000 units penicillin G per plate and 0.3 g/L Rose Bengal.
Ten plates were used for each plant part. The plates were then incubated at
27ºC for 7-15 days