Chlorophyll and carotenoids were extracted from 10mg (wet
plant biomass) of floating leaves with 2mL of dimethyl sulfoxide.
Samples were incubated in darkness for 12h at 45◦C. Chlorophyll
a, band carotenoids were determined spectrophotometrically at
wavelengths of 665, 649 and480nm. Pigment concentrationswere
calculatedusingWellburn’s formulae[36], andexpressedasgg−1
FW