A perusal of available literature reveals that micropropagation has been achieved using immature or mature embryos, shoot tip explants and from axenic nodel segments in Dendrobium aphyllum (Roxb.) C.E.C.Fisch., D. candidum Wall. ex Lindl. and other hybrids of Dendrobium (Zhang et al. 1993, Liu et al. 1988, Shiau et al. 2005, Xie et al. 2010, Nambiar et al. 2012). However, there is no report on clonal propagation of D. aggregatum using different explant sources. In this study, authors report the development of an efficient simple and reproducible one step protocol for multiple D. aggregatum seedlings, rooting of the microshoots and successful transplantation.