The laboratory population of B. tryoni was initially supplied as pupae by the Department of Employment, Economic Development and Innovation (DEEDI), Indooroopilly , Queensland.
Thereafter, it was reared following the general technique of Heather and Corcoran (1985) at the University of Queensland Gatton Campus. Adults were kept in 50 cm × 50 cm × 50 cm aluminium-framed cages in a room maintained at 25 ± 1°C,RH 60 ± 10%, and 14:10 (light : dark) photoperiod. Adultswere provided with water, sugar cubes and yeast hydrolysate (MP Biomedicals). Yeast hydrolysate (40 g) was mixed with water (12 mL) to form a paste and then scraped onto a piece of cardboard.