following cycling protocol: 5 min at 95C, followed by 35 cycles consisting of 94C for 30 s, 60C for 30 s, 72C for 30 s, and with a final extension of 72C for 10 min.
following cycling protocol: 5 min at 95C, followed by 35 cyclesconsisting of 94C for 30 s, 60C for 30 s, 72C for 30 s, and witha final extension of 72C for 10 min.
following cycling protocol: 5 min at 95C, followed by 35 cycles consisting of 94C for 30 s, 60C for 30 s, 72C for 30 s, and with a final extension of 72C for 10 min.