2.5. Effect of temperature on cell growth and EPS production
Having identified the optimum casein hydrolysate level to be at
1.5% for both Bifidobacterium strains, milk fermentations were carried
out with 1.5% casein hydrolysate supplementation at 25, 30, 37 and
42 °C as described in Section 2.2, for 48 h. Samples were collected
aseptically for enumeration of cells, to establish growth curves, and
quantify the EPS.