Twenty nine isolates of S. pyogenes belonged to 19 patients, were tested for biofilm production by using Microtiter-plate method. Also, Modified Microtiter-plate method was conducted to study the effect of different vinegar types (Date, Apple, and Grape) as anti-biofilm, in vitro. Out of 75.9% produced biofilm according to depending biofilm criteria, while 24.1% were non-biofilm producers