the total plate count analysis was done to the Sni protocol __with some modifications as followed.
homogenized fish sample 2in 10 - physiological solution -- was prepared as the initial dilution
The serial dilution was made by adding 0.1of previous dilution into 0.9 . physiological solution
the resulting diltions__10^6 were spread streaked onto plate count agar (pca) plates using autoclaved cotton buds