This research aimed to analyse Coliform bacteria in the ice that selling at Rajabhat Mahasarakham University and compare contamination Coliform bacteria in the ice each location at Rajabhat Mahasarakham University between August (2015) to June (2016). The participants were 2 stores at canteen of the University, 2 stores at canteen area at Student Affaires, 3 smooth shops at the University and 5 shops at market at Rajabhat Mahasarakham University. Research instrument in cluded trail ware by Department of Medical Sciences at Ministry of Public Health