The analyses of TAN excretion rates were obtained under farmlike
conditions. TAN excretion rates for the RAS1 and RAS2 fish
groups were evaluated under normal feeding and starvation conditions.
The juveniles were deprived of feed for 48 h before carrying
out the fasting excretion rate experiments.
TANmeasurements were obtained by taking 24 samples per day,
with an hour between each sample. The groups were replicated
in three cycles of 24 h (3 days for 14 ◦C with feeding; 3 days for
18 ◦C with feeding; 3 days for 14 ◦C deprived of feed; and 3 days
for 18 ◦C deprived of feed). Hence TAN excretion was tested in the
same tank with the same biomass during three consecutive days
for each experiment