Procedures;
1.Weight approximately 5 g of dried grinded carrots into thimble.
2.150ml of Hexane is poured into the 250 ml round bottom flask,and boiling chips were placed.
3.Soxhlet apparatus is set carefully for the extraction as shown in Figure 1. the water in-line should be at the bottom line, whereas the water out-line is the upper line for cooling system.
4.place the round bottom flask with soxhlet apparatus in oil bath. turn on the heater and alow the extraction is process for 1.5 hours at reflux temperature.
5.After about and a half an hour of extraction, let the soxhlet apparatus to be cool. collect the b-carotene extract and dried the extract with sodium sulphate.
6.Determination the absorption of b-carotene by UV-Visible technique.