Immunodiagnosis of fasciolosis in animals by antibody detection has been developed (Zimmerman et al., 1985; Swarup et al., 1987; Fagbemi and Obarisiagbon, 1990; Guobadia and Fagbemi, 1995; Sriveny et al., 2006); however, the presence of antibodies is not a direct indicator of active infection, and cross-reactivity with other parasites’ antigens is often difficult to differentiate.