Analysis of carotenoids content
Carotenoid content
was
determined using acetone-petroleum
ether extraction followed by spectrophotometric analysis, as
described by Rodriguez-Amaya, and Kimura (2004). Extraction of
carotenoids was performed by grinding the raw or boiled potatoes
in a mortar and pestle, filtration through a sintered glass filter
under vacuum, and separation of the carotenoids from acetone into
petroleum ether.