be used for a more detailed analysis of Lactobacillus species
because sequences are less conserved than the 16S rRNA gene
sequence (31).
The CV values (0.04 to 0.14) for the different species-specific
duplex 5 nuclease assays are acceptable and comparable to the
CV values (0.09 to 0.28) reported earlier for determination of
bacteria in fecal samples with the FISH technique (12, 18)