Abstract
Sapodilla seed coat was extracted into n-hexane, EtOAc, and aqueous fractions (0.041, 0.085, and 1.124% (w/w)). The EtOAc fraction was the most potent antioxidant (IC50DPPH = 2.387 ± 0.023, and IC50ABTS = 4.468 ± 0.043 μg/ml), and was significantly (p < 0.05) more potent than ascorbic acid (IC50DPPH = 3.130 ± 0.039, and IC50ABTS = 6.288 ± 0.098 μg/ml) with lipid peroxidation, and tyrosinase inhibitory effects (IC50 = 40.020 ± 0.418, and 138.553 ± 0.256 μg/ml). The total phenolic content positively correlated with DPPH•, and ABTS•+ (r = 0.999 and 0.997) scavenging activities. Gallic acid, chlorogenic acid, and quercetin were identified in the fractions. Quercetin was antioxidant responsive (rDPPH = 0.999 and rABTS = 0.997), and positively correlated with total phenolic content (r = 1). The EtOAc fraction was non-cytotoxic to Vero cells, demonstrating that sapodilla seed coat extract could serve as a promising multifunctional ingredient in natural cosmetics.