Preparation of solution of Naproxen at
different concentrations and scanning
wavelength at different media
50μg/ml of stock solution was prepared by
dissolving 5mg of Naproxen in 100ml of
phosphate buffer having pH of 2.5 and pH 7.4
respectively, in 0.1N HCl and in Distilled water.
Standard solution of various concentrations (1,2, 3, 4, 5μg/ml) were prepared by dilution from
each stock solution. The wavelength of
maximum emission (λmax) of Ciprofloxacin in
each media were found by scanning them over
the UV range of 190nm to 400nm. Concentration
vs. absorbance was plotted and standard curve
was prepared by scanning the wavelength (λmax)
of Naproxen at 228nm in the media of Buffer pH
7.4, in the media of 0.1N HCl and in the media
of Distilled water presented in Figure 3.