The proliferated globular embryos in flasks were transferred to
40 mL MS solid medium supplemented with GA 3 and 3% sucrose in
100 mm ? 20 mm plastic petri dishes for maturation and germi-
nation. To investigate the effect of GA 3 concentration on maturation
and germination of somatic embryos, 150 globular embryos were
transferred to germination medium containing 0 mg/L, 1 mg/L,
3mg/L, 5mg/L, 7mg/L, or10mg/L ofGA 3 .Culturesweremaintained
at 23 ? 2 ? C under dim light illumination (12 m mol/m 2 /s) with a 16/
8 h (light/dark) photoperiod. After 6 wk of culture, maturation and
germination of embryos were examined. The experiment was
repeated three times.